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Genetics

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Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

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This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.

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Tumorsphere Derivation Treatment Primary Tumor Cells Mouse Rhabdomyosarcomas Soft-tissue Sarcomas Histologically Myogenic Markers Cell Of Origin Reproducible Methods Reliable Methods Freshly Harvested Tumor Tissues Tumorsphere Formation Assay Advantages Cellular Properties Expansion Enrichment Specific Cell Population Spheroid Structure Tumor Environment Drug-screening Studies Optimization Of Culture Conditions
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