Visualizzazione e analisi del trasporto intracellulare di organelli e di altri carichi in Astrociti
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August 28, 2019
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Summary
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Qui descriviamo un metodo in vitro di imaging dal vivo per visualizzare il trasporto intracellulare di organelli e il traffico di proteine della membrana plasmatica negli astrociti murini. Questo protocollo presenta anche una metodologia di analisi delle immagini per determinare gli itinerari e la cinetica nel trasporto merci.
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Cite this Article
Creighton, B. A., Ruffins, T. W., Lorenzo, D. N. Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes. J. Vis. Exp. (150), e60230, doi:10.3791/60230 (2019).
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