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Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups
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Neuroscienze
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JoVE Journal Neuroscienze
Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

Reliable Isolation of Central Nervous System Microvessels Across Five Vertebrate Groups

DOI:
10.3791/60291-v

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10:35 min

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January 12, 2020

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, , , ,
1Department of Anatomy, Physiology and Cell Biology,University of California Davis, 2University of Veterinary Medicine Hannover Foundation

Capitoli

  • 00:04Titolo
  • 00:40Central Nervous System (CNS) Tissue Dissection
  • 02:35CNS Tissue Homogenization
  • 04:20Microvessel Purification
  • 05:57Microvessel Elution and Filtration
  • 07:44Results: Representative Microvessel Protein Expression
  • 09:57Conclusion

Summary

Traduzione automatica

Traduzione automatica

The goal of this protocol is to isolate microvessels from multiple regions of the central nervous system of lissencephalic and gyrencephalic vertebrates.

Tags

Microvessel IsolationCentral Nervous SystemVertebratesDissectionMeningesChoroid PlexusHomogenization

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