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Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Article DOI: 10.3791/60304-v • 07:21 min • December 26th, 2019
December 26th, 2019 •
1, 2, 3, 1, 4, 1
1Medical Oncology Department, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), CIBERONC, 2Department of Medical Oncology, Hospital Universitario Puerta de Hierro de Majadahonda, 3Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas - Universidad Autónoma de Madrid, CIBERONC, 4Department of Oncology & Pathology, Karolinska Institutet

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The aim of this method is to obtain fibroblast-derived 3D matrices as a natural scaffold for subsequent cellular assays. Fibroblasts are seeded in a pre-treated culture plate and stimulated with ascorbic acid for matrix generation. Matrices are decellularized and blocked to culture relevant cells (e.g., endothelial cells).

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Fibroblast-derived 3D Matrix System Endothelial Tube Formation Assay Cultures Interactions Extra-cellular Matrix Advantage Regenerated In Vivo Fibroblast Baking Dishes Matrix Effect Persistent Situation Physical Studies Survivors Matrix Properties Visualization Pipetting Technique Regent Gelatin Glutaraldehyde Ethanolamine Solutions Ascorbic Acid Lysis Buffer PBS Pen-strep DMEM With 10%FBS Heat-denatured 2%BSA
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