Produzione di Dynein e Kinesin Motor Ensemble su nanostrutture DNA Origami per l'osservazione a singola molecola
6,602 Views
•
08:09 min
•
October 15, 2019
DOI:
Summary
Automatically generated
L'obiettivo di questo protocollo è quello di formare insiemi di motori molecolari sulle nanostrutture di origami del DNA e osservare la motilità dell'insieme utilizzando la microscopia a fluorescenza totale della riflessione interna.
Video correlati
07:59
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Video correlati
14601 Views
13:32
Designing a Bio-responsive Robot from DNA Origami
Video correlati
22306 Views
09:32
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Video correlati
6470 Views
08:04
Assembling Molecular Shuttles Powered by Reversibly Attached Kinesins
Video correlati
6901 Views
08:59
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Video correlati
11565 Views
10:46
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
Video correlati
2587 Views
08:16
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Video correlati
1953 Views
07:44
Design and Synthesis of a Reconfigurable DNA Accordion Rack
Video correlati
7119 Views
14:43
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Video correlati
11604 Views
07:47
Directly Measuring Forces Within Reconstituted Active Microtubule Bundles
Video correlati
1654 Views
Read Article
Cite this Article
Hu, J., Derr, N. D. Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation. J. Vis. Exp. (152), e60369, doi:10.3791/60369 (2019).
Copy