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Journal / Developmental Biology / Fluorescent Calcium Imaging and Subsequent In…
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Developmental Biology

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JoVE Journal Developmental Biology
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
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Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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We present a two-part protocol that combines fluorescent calcium imaging with in situ hybridization, allowing the experimenter to correlate patterns of calcium activity with gene expression profiles on a single-cell level.

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Fluorescent Calcium Imaging In Situ Hybridization Neuronal Precursor Characterization Xenopus Laevis Calcium Activity Patterns Gene Expression Single Cell Level UV Sterilizing Plastic Petri Dishes Cell Culture Dish Calcium Solution Calcium And Magnesium Free Solution 0.1X MMR Supplemented With Gentamicin Collagenous B Dissecting Microscope Embryos
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