Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in <em>Xenopus laevis</em>

Eileen  F. Ablondi; Sudip Paudel; Morgan Sehdev; John  P. Marken; Andrew  D. Halleran; Atiqur Rahman; Peter Kemper; Margaret S. Saha

doi:10.3791/60726

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Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in <em>Xenopus laevis</em>

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis

Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis

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7,934 Views

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00:09 min

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February 18, 2020

DOI:

10.3791/60726-v

DOI:

10.3791/60726-v

•

00:09 min

February 18, 2020

•

7912 Views

Eileen F. Ablondi¹, Sudip Paudel², Morgan Sehdev³, John P. Marken⁴, Andrew D. Halleran⁴, Atiqur Rahman⁵, Peter Kemper⁵, Margaret S. Saha²

¹Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University ²Department of Biology,College of William and Mary ³Harvard Medical School,Harvard University ⁴Department of Bioengineering,California Institute of Technology ⁵Department of Computer Science,College of William and Mary

Summary

Vi presentiamo un protocollo in due parti che combina l'imaging fluorescente del calcio con l'ibridazione in situ, permettendo allo sperimentatore di correlare i modelli di attività del calcio con i profili di espressione genica a livello unicellulare.