Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

David C. Montrose; Erin M. McNally; Erika Sue; Andrew J. Dannenberg

doi:10.3791/60949

Esecuzione di biopsie di avvicinamento delle dita guidate dal colonscopico nei topi e valutazione...

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Immunology and Infection

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JoVE Journal Immunology and Infection

Performing Colonoscopic-Guided Pinch Biopsies in Mice and Evaluating Subsequent Tissue Changes

Esecuzione di biopsie di avvicinamento delle dita guidate dal colonscopico nei topi e valutazione dei successivi cambiamenti tissutali

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08:52 min

February 5, 2021

DOI: 10.3791/60949-v

David C. Montrose^1,2, Erin M. McNally³, Erika Sue³, Andrew J. Dannenberg³

¹Department of Pathology, Renaissance School of Medicine,Stony Brook University, Stony Brook, NY, ²Stony Brook Cancer Center, Stony Brook, NY, ³Department of Medicine,Weill Cornell Medicine, New York, NY

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Overview

This article describes a method for inducing colonoscopic-guided pinch biopsies in mice, allowing for real-time tracking of wound closure. It also details tissue preparation for various analyses related to wound healing.

Key Study Components

Area of Science

Neuroscience
Biology
Wound Healing

Background

Colonic wound healing is crucial for understanding gastrointestinal diseases.
This method enables precise control over injury location and healing timing.
It can provide insights into inflammatory bowel disease (IBD) pathogenesis.
Visual demonstration is essential for successful technique execution.

Purpose of Study

To develop a reliable method for studying colonic wound healing.
To analyze molecular and histological changes post-injury.
To enhance understanding of IBD mechanisms.

Methods Used

Colonoscopic-guided pinch biopsy technique.
Real-time tracking of wound closure.
Tissue preparation for histological analysis.
Immunohistochemical and molecular analyses of the wound bed.

Main Results

Successful induction of controlled colonic injuries.
Real-time monitoring of the wound healing process.
Insights into the histological changes during healing.
Potential implications for IBD research.

Conclusions

This method is effective for studying colonic wound healing.
It provides a framework for future research on gastrointestinal diseases.
Training and practice are essential for successful technique application.

Frequently Asked Questions

What is the main advantage of this technique?

The main advantage is the precise control over the injury location and timing of the wound healing process.

What challenges might beginners face?

Beginners may struggle with inserting the colonoscope and creating consistent wounds.

How can one overcome difficulties with the technique?

Repetitive practice of the techniques can help overcome initial challenges.

Why is visual demonstration important?

Visual demonstration is critical due to the multiple coordinated steps and nuances involved in the procedure.

What types of analyses can be performed on the tissues?

Histological, immunohistochemical, and molecular analyses can be performed on the wound bed tissues.

What insights can this method provide?

It can provide insights into the molecular and histological changes that occur following colonic injury.

Qui, forniamo una descrizione dettagliata della procedura per indurre biopsie di pizzico guidate dal colonscopico nei topi e tracciare la chiusura delle ferite in tempo reale. Inoltre, vengono forniti metodi per la preparazione di tessuti per analisi istologiche, immunoistochimiche e molecolari del letto della ferita.

Questo metodo fornisce un approccio ideale per studiare la guarigione delle ferite coloniche e i cambiamenti molecolari e istologici che si verificano a seguito di lesioni. Questo metodo può anche far luce sulla patogenesi IBD. Il principale vantaggio di questa tecnica è controllare con precisione la posizione della lesione e la tempistica del processo di guarigione delle ferite.

Gli individui che eseguono questo metodo per la prima volta possono avere difficoltà a inserire il colonscopio nel colon del mouse e creare ferite coerenti. Praticare ripetutamente queste tecniche dovrebbe superare questi problemi. Dati i molteplici passaggi coordinati e le sfumature coinvolte in questa procedura, la dimostrazione visiva di questo metodo è fondamentale.

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