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Biochemistry

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Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Article DOI: 10.3791/61005-v • 06:07 min • June 7th, 2020
June 7th, 2020 •
1, 1, 1
1Department of Anthropology, Indiana University

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We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.

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Phytoestrogens Cell-based Estrogen Receptor Assay Reporter Assay Plant Foods Phytosteroids Physiological Processes Behavior Reproductive Success Estrogenic Compounds Biological Activity Xenoestrogen Activity Medicine Cell Proliferation Primate Ecology Evolution Aseptic Technique Contamination Freeze-drying Lyophilizer Grinding Mill Mesh Screen Extraction Methanol Solution Aluminum Foil Orbital Shaker Filter Paper Drip Filtration System Rotary Evaporator
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