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Bioengineering

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JoVE Journal Bioengineering
In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding
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An Improved Method for the Preparation of Type I Collagen From Skin
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In Vitro Model of Human Cutaneous Hypertrophic Scarring using Macromolecular Crowding

Article DOI: 10.3791/61037-v • 08:20 min • May 1st, 2020
May 1st, 2020 •
1, 1, 1, 1, 1,2, 1, 1,3, 1
1Skin Research Institute of Singapore, Agency for Science, Technology and Research (A*STAR), 2School of Chemical and Life Sciences, Singapore Polytechnic, 3Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR)

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This protocol describes the use of macromolecular crowding to create an in vitro human hypertrophic scar tissue model that resembles in vivo conditions. When cultivated in a crowded macromolecular environment, human skin fibroblasts exhibit phenotypes, biochemistry, physiology, and functional characteristics resembling scar tissue.

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In Vitro Model Human Cutaneous Hypertrophic Scarring Macromolecular Crowding Collagen-rich Model Crowded Molecular Environment Scar Tissues Extracellular Matrix Monolayer Cell Culture System Scar-derived Fibroblasts Normal Fibroblasts 24 Well Plates Medium Ficoll 70 Ficoll 400 Ascorbic Acid MMC Media Water Bath Sterilize 0.2 Micrometer Filter Sirius Red Solution
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