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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells
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Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Article DOI: 10.3791/61166-v • 09:35 min • May 19th, 2020
May 19th, 2020 •
1, 1,2, 1,2, 1, 1, 1
1Department of Neurology, Geisel School of Medicine, Dartmouth-Hitchcock Medical Center, 2Program in Experimental and Molecular Medicine, Dartmouth College

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This paper presents two optimized protocols for examining resident and peripherally derived immune cells within the central nervous system, including the brain, spinal cord, and meninges. Each of these protocols helps to ascertain the function and composition of the cells occupying these compartments under steady state and inflammatory conditions.

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Isolating Central Nervous System Tissues Meninges Immune Cell Analysis Single Cell Techniques Histological Methods CNS Resident Cells Microglia Astrocytes Pericytes Endothelial Cells Stromal Cells Neurons Brain And Spinal Cord Samples Euthanized Mice Midline Incision Nasal Bone Mandible Tongue Eyes External Auditory Meatus Rib Cage Spinal Column Decalcification
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