Analisi cinetica ad alta velocità ad alto rendimento parallela per cleavage del DNA specifico del sito
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May 06, 2020
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Viene descritto un metodo altamente parallelo per misurare la scissione specifica del sito del DNA a livello di singola molecola. Questo protocollo illustra la tecnica utilizzando la restrizione endonuclease NdeI. Il metodo può essere facilmente modificato per studiare qualsiasi processo che si traduce in scissione del DNA specifica del sito.
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Cite this Article
Matozel, E. K., Dale, N., Price, A. C. Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage. J. Vis. Exp. (159), e61236, doi:10.3791/61236 (2020).
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