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DOI: 10.3791/61341-v
Xinyi Liu1,2, Si Wu1,2, Wenqian Li1,2, Meihong Zhang1,2, Yan Wu1,2, Ning Zhou3, Pa Wu1,2
1Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Science,Hunan Normal University, 2State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory for Microbial Molecular Biology, College of Life Science,Hunan Normal University, 3Department of Infectious Diseases, the Second Xiangya Hospital,Central South University
Please note that some of the translations on this page are AI generated. Click here for the English version.
This study investigates the complex community of gut bacteria in mosquitoes and their impact on host physiology. The protocol includes isolating and identifying specific gut bacteria, depleting them with antibiotics, and reintroducing selected strains to assess their effects on mosquito health.
Questo articolo presenta un protocollo per studiare l'effetto dei singoli batteri dell'intestino di zanzara, tra cui l'isolamento e l'identificazione di microbi cultivable delle zanzare, l'esaurimento antibiotico dei batteri dell'intestino di zanzara e reintrodurre una specie di batteri specifici.
Mosquito midgut ospita una complessa comunità di microbi che influenzano il metabolismo dell'ospite, la riproduzione, la forma fisica e la competenza veterinaria. Con ogni microbo intestinale è un effetto diverso. Questo video introduce la procedura per studiare il rispettivo ruolo di ogni ceppo batterico o fisiologia ospite.
Per acquisire una coltura di colonie batteriche intestinali, sezionare il midgut della zanzara in condizioni sterili. Macinate la midgut sezionata, serotologinate e sparpanate sul piatto di agar. Scegli singole colonie dalla piastra e identifica le specie di batteri attraverso la loro sequenza genica di 16 SRNA.
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