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Biochemistry

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JoVE Journal Biochemistry
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Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Article DOI: 10.3791/61407-v • 05:22 min • January 25th, 2022
January 25th, 2022 •
1, 1,2, 1,2
1Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, 2Proteins Easy Corp

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This protocol allows for the preparation of transverse sections of cereal seeds (e.g., rice) for the analysis of endosperm and starch granule morphology using scanning electron microscopy.

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Transverse Sectioning Mature Rice Kernels Scanning Electron Microscopy Imaging Pipette Tips Immobilization Support Starch Granules Visualization Plastic Cone Dry Tissues And Organs Cheap And Easy Manifold Forensic Microscopy Advantage Resistant Starch Enzymes Accessibility Amylolysis Glycemic Index Glucose Spikes Breeding Better Rice Varieties Type 2 Diabetes Dr. Kiah Barton Faculty Core Imaging Facility De-husking Rubber Stopper Abrading Kernel Removing Husks Endosperm Shattering
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