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Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Article DOI: 10.3791/61421-v • 09:05 min • June 29th, 2020
June 29th, 2020 •
1, 1, 2, 1, 1, 1, 1, 1, 1, 1
1Life Sciences Division, National Research Council of Canada, 2Galenvs Sciences, Inc.

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Epigenetic markers are used for white blood cell (WBC) subtyping through the quantification of DNA methylation patterns. This protocol presents a multiplex droplet polymerase chain reaction (mdPCR) method using a thermoplastic elastomer (TPE)-based microfluidic device for droplet generation allowing for precise and multiplex methylation-specific target quantification of WBC differential counts.

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Methylation Specific Multiplex Droplet PCR Polymer Droplet Generator Device Hematological Diagnostics Differential Leukocyte Counting Epigenetic Methylation Markers MdPCR Immunofluorescent Staining Methods Fresh Blood Samples Costly Antibodies Infection Inflammation Anemia Leukemia Early Prognostic Cancer Biomarker Protein Kinase K Lysis Binding Buffer Magnetic Beads Human Peripheral Blood Mononuclear Cells PBS Wash Buffer
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