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Quantification of Cell-Substrate Adhesion Area and Cell Shape Distributions in MCF7 Cell Monolayers

Article DOI: 10.3791/61461-v • 06:46 min • June 24th, 2020
June 24th, 2020 •
1, 1, 1, 1, 1, 1
1Maria Sklodowska-Curie National Research Institute of Oncology

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The article describes quantification of 1) the size and number of focal adhesions and 2) cell shape index and its distribution from confocal images of the confluent monolayers of MCF7 cells.

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Quantification Cell-substrate Adhesion Cell Shape MCF7 Cell Monolayers Intracellular Structure Two-dimensional Image Confocal Microscope Opensource Software Seeding Culture Medium Collagen One Coated Chamber Slide Incubator Optical Inverted Microscope Z-slice Images Focal Adhesion Analysis ImageJ Image Scale Pixels Filename Region Of Interest Measurement Options Background Subtraction Rolling Ball Radius Sliding Paraboloid
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