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Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants

Article DOI: 10.3791/61517-v • 08:33 min • August 5th, 2020
August 5th, 2020 •
1,2, 1,2, 1, 1,2,3, 1,2,3, 1,3
1Department of Animal, Plant and Soil Science, AgriBio Building, La Trobe University, 2Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, 3Australian Research Council Research Hub for Medicinal Agriculture, AgriBio Building, La Trobe University

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Presented here is a protocol for laser-capture microdissection (LCM) of plant tissues. LCM is a microscopic technique for isolating areas of tissue in a contamination-free manner. The procedure includes tissue fixation, paraffin embedding, sectioning, LCM and RNA extraction. RNA is used in the downstream tissue-specific, temporally resolved analysis of transcriptomes.

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Laser-Capture Microdissection RNA-Sequencing Spatial Gene Expression Analysis Temporal Gene Expression Analysis Tissue-specific Gene Expression Analysis Plants Laser Technique Visualization Of Cells Isolation Of Cells Contact-free Isolation Sample Preparation Tissue Fixation Embedding Barley Seed Fixative Vacuum Infiltration Tissue Processing
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