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Journal / Developmental Biology / Drosophila Embryo Preparation and Microinjection…
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Developmental Biology

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JoVE Journal Developmental Biology
Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer
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Drosophila Embryo Preparation and Microinjection for Live Cell Microscopy Performed using an Automated High Content Analyzer

Article DOI: 10.3791/61589-v • 05:52 min • January 19th, 2021
January 19th, 2021 •
1,2, 2, 1
1Department of Biology, San Francisco State University, 2Department of Biochemistry & Biophysics, UCSF Mission Bay

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Presented here is a protocol to microinject and simultaneously image multiple Drosophila embryos during embryonic development using a plate-based, high content imager.

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Drosophila Embryo Preparation Microinjection Live Cell Microscopy Automated High Content Analyzer Sample Preparation Multi-point Acquisition Inverted Microscope Embryo Alignment Ventral Side Orientation Heptane Glue Coverslip Spacer Razor Blade Stainless Steel Spatula 3.5 Centimeter Dish Stereo Microscope Halocarbon Oil
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