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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualisatie van Pseudomonas aeruginosa in het sputum van patiënten met cystische fibrose
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Visualisatie van Pseudomonas aeruginosa in het sputum van patiënten met cystische fibrose

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Dit protocol biedt methoden voor visualisatie van bacteriële cellen en polysaccharidesyntheselocus (Psl) polysaccharide in het sputum van cystische fibrosepatiënten.

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Immunologie en infectie Nummer 161 Chronische infectie Longfunctie Morbiditeit Antibiotische therapie Pediatrische patiënten Ruimtelijke organisatie Aggregatie Biofilmvorming Polysaccharideproductie Sputummonsters Weefselopruimingstechniek Hydrogelmatrix 3D-structuren Fluorescerende labels Fluorescentie In Situ Hybridisatie Anti-PSL-antilichamen Exopolysaccharide DAPI-kleuring Gastheercellen Beeldvorming met hoge resolutie
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