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Journal / Biology / Visualization of Replisome Encounters with an…
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Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion
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Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.

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Visualization Replisome Encounters Antigen-tagged Blocking Lesion DNA Adduct Immunodetection Protein-DNA Interactions Cell Cycle-dependent Interactions In Situ Visualization Ishani Majumdar Seidman Laboratory Cell Culture Dishes Cell Adhesive Solution Dig TMP-supplemented Medium Equilibration UV Exposure UVA Light Dose Culture Medium PBS
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