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Journal / Biochemistry / Labelling and Visualization of Mitochondrial…
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JoVE Journal Biochemistry
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Labelling and Visualization of Mitochondrial Genome Expression Products in Baker's Yeast Saccharomyces cerevisiae

Article DOI: 10.3791/62020-v • 08:33 min • April 11th, 2021
April 11th, 2021 •
1,2, 1, 1, 1, 3, 1
1Department of Biology, M.V. Lomonosov Moscow State University, 2Institute of Functional Genomics, M.V. Lomonosov Moscow State University, 3National Research Centre "Kurchatov Institute"

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Baker’s yeast mitochondrial genome encodes eight polypeptides. The goal of the current protocol is to label all of them and subsequently visualize them as separate bands.

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Labelling Visualization Mitochondrial Genome Expression Products Baker's Yeast Saccharomyces Cerevisiae Aerobic Respiration Human Disease Circular Genome Radioactive Labeling Translational Products Gel Electrophoresis Protocol Stock Cultures Medium Incubator Optical Density Absorbency Units Sterile Tubes Yeast Cells Supernatant Water Wash Wipe Agar Medium
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