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Journal / Biochemistry / Imaging of Podocytic Proteins Nephrin, Actin, and …
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Biochemistry

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Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Article DOI: 10.3791/62079-v • 06:18 min • April 23rd, 2021
April 23rd, 2021 •
*1, *1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University
* These authors contributed equally

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The presented method enables visualization of fluorescently labeled cellular proteins with expansion microscopy leading to a resolution of 70 nm on a conventional microscope.

Tags

Expansion Microscopy Glomerular Proteins Nanoscale Resolution Conventional Microscope Glass Cover Slips Gelation Chamber Double Distilled Water Glass Slide Staining Chamber Anchoring Buffer Immune Stained Cells Substrate
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