Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / A Plate-Based Assay for the Measurement of…
JoVE Journal
Neuroscience

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Neuroscience
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Journal (Neuroscience)
A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in Blood
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
Journal (Neuroscience)
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
 

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

Article DOI: 10.3791/62127-v • 07:56 min • August 11th, 2021
August 11th, 2021 •
*1, *2, 3, 2
1Department of Medicine, School of Medicine, Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences, City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics, University of Florida College of Medicine
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Tags

Plate-based Assay Measurement Endogenous Monoamine Release Acute Brain Slices Adaptation Radiolabeled Monoamines Pharmacological Conditions Cost-effective Vesicular Release Transporter Mediated Release Brain Slices Dissection Buffer Coronal Brain Sections Dorsal Striatum Hippocampus Control Slices Experimental Slices Punches Plastic Transfer Pipette
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter