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Comparative Analysis of Experimental Methods to Quantify Animal Activity in Caenorhabditis elegans Models of Mitochondrial Disease

Article DOI: 10.3791/62244-v • 05:51 min • April 4th, 2021
April 4th, 2021 •
*1, *1, 1, 1,2, 1,2
1Mitochondrial Medicine Frontier Program, Division of Human Genetics, Department of Pediatrics, The Children’s Hospital of Philadelphia, 2Department of Pediatrics, University of Pennsylvania Perelman School of Medicine
* These authors contributed equally

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This study presents protocols for two semi-automated locomotor activity analysis approaches in C. elegans complex I disease gas-1(fc21) worms, namely, ZebraLab (a medium-throughput assay) and WormScan (a high-throughput assay) and provide comparative analysis among a wide array of research methods to quantify nematode behavior and integrated neuromuscular function.

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Comparative Analysis Experimental Methods Quantify Animal Activity C. Elegans Models Mitochondrial Disease Semiautomated Assays Locomotor Activity Drug Screening Reproducibility Motility Assays Neuromuscular System Glass Slides Worm Pick Stereomicroscope S.basal Solution Technical Replicates Acclimate Swim Activity
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