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Biochemistry

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JoVE Journal Biochemistry
Preparação de Lamelas a partir de Amostras Biológicas Vítreas Usando um Microscópio Eletrônico de Varredura de Feixe Duplo para Tomografia Crio-Eletrônica
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Journal (Biochemistry)
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Journal (Biochemistry)
Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
 
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Preparação de Lamelas a partir de Amostras Biológicas Vítreas Usando um Microscópio Eletrônico de Varredura de Feixe Duplo para Tomografia Crio-Eletrônica

Article DOI: 10.3791/62350-v • 07:00 min • August 5th, 2021
August 5th, 2021 •
1,2, 3,4, 3, 1, 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University

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Usando fresagem por feixe de íons focalizado para produzir lamelas vítreas on-grid a partir de amostras biológicas congeladas por imersão para tomografia crio-eletrônica.

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Retração FIB-milling cryoFIB-SEM lamela tomografia cryoET cryoEM Plasmodium falciparum
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