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Immunology and Infection

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Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Article DOI: 10.3791/62488-v • 07:44 min • August 12th, 2021
August 12th, 2021 •
1,2, 1,2, 1,2, 1,2, 3, 1,2, 1,2, 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 3Department of Biotechnology, University of Tehran

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The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

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SARS-CoV-2 Receptor-Binding Domain Antibody HiBiT-Based Bioreporter COVID-19 Research Assay Anti-SARS-CoV-2 Antibodies Patient Serum Samples Serological Assays Sensitivity Response Time Cell Culture Dulbecco's Modified Eagle Medium Fetal Bovine Serum Penicillin Streptomycin Biological Safety Cabinet 70% Ethanol Cell Line Petri Dish Confluency Level
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