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Reconstruct Human Retinoblastoma In Vitro
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JoVE Journal Ricerca sul cancro
Reconstruct Human Retinoblastoma In Vitro

Reconstruct Human Retinoblastoma In Vitro

1,195 Views

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06:52 min

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October 11, 2022

DOI:

10.3791/62629-v

DOI:
10.3791/62629-v

•

06:52 min
October 11, 2022

•

3 Views
,
1Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital,Capital Medical University, Beijing Ophthalmology & Visual Science Key Laboratory

Summary

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We describe a method for generating human retinoblastoma (RB) by introducing biallelic RB1 mutations in human embryonic stem cells (hESC). RB cell lines could also be successfully cultured using the isolated RB in a dish.

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Cite this Article

Zhang, X., Jin, Z. Reconstruct Human Retinoblastoma In Vitro. J. Vis. Exp. (188), e62629, doi:10.3791/62629 (2022).

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