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Biochemistry

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JoVE Journal Biochemistry
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
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Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes

Article DOI: 10.3791/62904-v • 11:03 min • September 9th, 2021
September 9th, 2021 •
*1, *1, 1
1Department of Biochemistry, School of Medicine, West Virginia University
* These authors contributed equally

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Fatty acid β-oxidation is an essential metabolic pathway responsible for generating energy in many different cell types, including hepatocytes. Here, we describe a method to measure fatty acid β-oxidation in freshly isolated primary hepatocytes using 14C-labeled palmitic acid.

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Fatty Acid Oxidation Mitochondrial Beta-oxidation Peroxisomal Beta-oxidation Primary Mouse Hepatocytes Intact Cells Gene Expression Surgery Cannulation Perfusion Abdomen Chest 70% Ethanol Forceps Diaphragm Organs Inferior Vena Cava (IVC) Pump Buffer One Needle Portal Vein Flow Rate
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