Umore acqueo come biopsia liquida per retinoblastoma: paracentesi corneale chiara e analisi genomica

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Citato da 36

06:04 min.

7 settembre 2021

10.3791/62939-v

7 settembre 2021

4.1K visualizzazioni

Mary E. Kim¹^,² , Liya Xu¹^,²^,³^,¹¹ , Rishvanth K. Prabakar⁴ , Lishuang Shen⁵ , Chen-Ching Peng¹^,³ , Peter Kuhn³^,⁶^,⁷^,⁸ , Xiaowu Gai⁵^,⁹ , James Hicks³^,⁶^,¹⁰ , Jesse L. Berry¹^,²^,⁶^,¹¹

¹The Vision Center, Children’s Hospital Los Angeles, ²USC Roski Eye Institute, Keck School of Medicine of the University of Southern California, ³Department of Biological Sciences, Dornsife College of Letters, Arts, and Sciences, University of Southern California, ⁴Department of Molecular and Computational Biology, University of Southern California, ⁵Center for Personalized Medicine, Department of Pathology and Laboratory Medicine, Children’s Hospital Los Angeles, ⁶Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, ⁷Department of Aerospace and Mechanical Engineering, Viterbi School of Engineering, University of Southern California, ⁸Department of Biomedical Engineering, Viterbi School of Engineering, University of Southern California, ⁹Department of Pathology and Laboratory Medicine, Keck School of Medicine of USC, ¹⁰Department of Biochemistry and Molecular Medicine, Keck School of Medicine, University of Southern California, ¹¹The Saban Research Institute, Children’s Hospital Los Angeles

L'umore acqueo è una biopsia liquida ad alto rendimento per il retinoblastoma, un cancro intraoculare che non può essere sottoposto a biopsia in vivo a causa del rischio di diffusione extraoculare. In questo articolo, viene presentato un metodo per estrarre in sicurezza l'umore acqueo tramite paracentesi corneale chiara e i passaggi per l'analisi genomica per identificare i biomarcatori prognostici.