Rivista
/
Neuroscienze
/
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures
JoVE Journal
Neuroscienze
This content is Free Access.
JoVE Journal Neuroscienze
In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

In Situ Visualization of Axon Growth and Growth Cone Dynamics in Acute Ex Vivo Embryonic Brain Slice Cultures

DOI:
10.3791/63068-v

•

10:45 min

•

October 14, 2021

•

, ,
1Laboratory of Axon Growth and Regeneration,Deutsches Zentrum für Neurodegenerative Erkrankungen (DZNE)

Capitoli

  • 00:05Introduction
  • 01:11Embryo Extraction
  • 01:44Ex Utero and In Utero Electroporation
  • 03:35Brain Extraction and Embedding in Agarose
  • 04:39Organotypic Slice Culture
  • 05:43Image Acquisition and Data Analysis
  • 08:29Results: In Situ Axon Growth and Growth Cone Dynamics
  • 09:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol demonstrates a straightforward and robust method to study in situ axon growth and growth cone dynamics. It describes how to prepare ex vivo physiologically relevant acute brain slices and provides a user-friendly analysis pipeline.

Tags

Axon GrowthGrowth Cone DynamicsEx VivoEmbryonic Brain Slice CulturesIn Situ VisualizationDNA DeliveryMouse Embryonic BrainOrganotypic CultureAxon PlasticityTraumatic ConditionsPathological ConditionsElectroporationBrain DissectionSlicingEx Utero ElectroporationIn Utero ElectroporationLateral VentriclePlatinum Tweezer Electrodes

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

Functional Neuroimaging Using Ultrasonic Blood-brain Barrier Disruption and Manganese-enhanced MRI

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

Ex Vivo Preparations of the Intact Vomeronasal Organ and Accessory Olfactory Bulb

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

Slice It Hot: Acute Adult Brain Slicing in Physiological Temperature

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System

Read Article