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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Article DOI: 10.3791/63121-v • 12:26 min • January 29th, 2022
January 29th, 2022 •
1, 2, 2, 1, 3, 3, 2, 1
1Department of Cellular Biophysics, Max Planck Institute for Medical Research, 2Institute for Theoretical Physics and BioQuant, Heidelberg University, 3Institute for Molecular Systems Engineering (IMSE), Heidelberg University

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Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same sample.

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Hydrogel Patterning Techniques Cell Adhesion Control Traction Force Microscopy Methacrylated Glass Cover Slip Polyacrylamide Hydrogel Fluorescent Beads Micro-pattern Cover Slip Matrix Proteins Polymerize Fluorescence Microscopy Imaging Extracellular Matrix Cellular Traction Forces Light-induced Release Of Cells Experimental Observations Joel Christian Double Distilled Water Acetic Acid Trimethoxysilylpropyl Methacrylate Ethanol
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