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Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Article DOI: 10.3791/63227-v • 07:53 min • October 21st, 2021
October 21st, 2021 •
1, 2, 1, 2, 1
1Laboratory of Systems Neurobiology and Medicine, Division of Biological Science, Nara Institute of Science and Technology, 2Laboratory of Data-Driven Biology, Division of Biological Science, Nara Institute of Science and Technology

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To advance, growth cones must exert traction forces against the external environment. The generation of traction forces is dependent on actin dynamics and clutch coupling. The present study describes methods for analyzing actin dynamics, clutch coupling and traction forces for growth cone advance.

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Analyses Actin Dynamics Clutch Coupling Traction Force Growth Cone Advance Single Speckle Imaging Traction Force Microscopy Molecular Machineries Neurons Tetramethyl-rhodamine (TMR) Ligand Culture Medium Incubation Wash Leibovitz's L-15 Medium Epifluorescence Microscope Glass Bottom Dish Image Acquisition Parameters Exposure Time Lifeact HaloTag-actin Fluorescence Channels Binning Time Interval Frames
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