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Journal / Genetics / ACT1-CUP1 Assays Determine the Substrate-Specific …
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Genetics

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JoVE Journal Genetics
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Article DOI: 10.3791/63232-v • 07:31 min • June 30th, 2022
June 30th, 2022 •
1
1College of Arts and Sciences, Northwest University

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The ACT1-CUP1 assay, a copper growth assay, provides a quick readout of precursor messenger RNA (pre-mRNA) splicing and the impact mutant splicing factors have on spliceosomal function. This study provides a protocol and highlights the customization possible to address the splicing question of interest.

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ACT1-CUP1 Assay Substrate-specific Sensitivities Spliceosomal Mutants Budding Yeast Pre-messenger RNA Splicing Splicing Factor Mutation Growth Phenotype Sensitivity Range Time Efficiency Stress-induced Growth Aberrations Copper Plates Leucine Dropout Growth Media Agar Stir Bar Yeast Nitrogen Base Dropout Mix Deionized Water Autoclave Sterilization Glucose Copper Sulfate Dilutions
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