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Isolation and Characterization of Intact Phycobilisome in Cyanobacteria

Article DOI: 10.3791/63272-v • 06:26 min • November 10th, 2021
November 10th, 2021 •
1, 1,2
1Department of Life Science, National Taiwan University, 2Institute of Plant Biology, National Taiwan University

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The current protocol details the isolation of phycobilisomes from cyanobacteria by centrifugation through a discontinuous sucrose density gradient. The fractions of intact phycobilisomes are confirmed by 77K fluorescent emission spectrum and SDS-PAGE analysis. The resulting phycobilisome fractions are suitable for negative staining of TEM and mass spectrometry analysis.

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Isolation Characterization Intact Phycobilisome Cyanobacteria Protocol Technique Small Scale Reliable Efficient Method Cell Culture OD At 750 Nanometers B-HEPES Medium Constant Stirring 30 Degree Celsius Exponential Growth Phase Centrifuge Supernatant Cell Pellets Potassium Phosphate Buffer PH Seven Wet Weight Electronic Balance Cell Suspension Glass Beads Bead Beater
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