Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation of Rat Adipose Tissue Mesenchymal Stem…
JoVE Journal
Developmental Biology
Author Produced

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Developmental Biology
Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Journal (Developmental Biology)
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
Journal (Developmental Biology)
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
 

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

Capitoli

Riepilogo
Automatic Translation

Adipose tissue-derived mesenchymal stem cells (Ad-MSCs) can be a potential source of MSCs that differentiate into insulin-producing cells (IPCs). In this protocol, we provide detailed steps for the isolation and characterization of rat epididymal Ad-MSCs, followed by a simple, short protocol for the generation of IPCs from the same rat Ad-MSCs.

Tags

Isolation Rat Adipose Tissue Mesenchymal Stem Cells Differentiation Insulin-producing Cells Protocol Red Adipose Tissue Dried Simple Methods Genetics Sources Bone Marrow Perinatal Tissue Cultural Characteristics Multi Potent Disease Treatment Lipoaspirates Yield Autologous Transplantation Allogenic Transplantation Isolation Protocol Characterization Protocol Epididymal Fat Collagenous Digestion Vascular Fraction Plastic Adherence CD Markers
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter