Rimozione di un sito di partenza traslazionale interno dall'mRNA pur mantenendo l'espressione della proteina a lunghezza intera
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05:48 min
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March 16, 2022
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Summary
Automatically generated
Il presente protocollo descrive una singola mutazione M213L in Gja1 che mantiene la generazione di Connexin43 a lunghezza intera ma impedisce la traduzione della più piccola isoforma GJA1-20k tradotta internamente.
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Cite this Article
Shimura, D., Hunter, J., Katsumata, M., Shaw, R. M. Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein. J. Vis. Exp. (181), e63405, doi:10.3791/63405 (2022).
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