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A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
JoVE Journal
Biologia
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JoVE Journal Biologia
A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels

DOI:
10.3791/63578-v

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04:30 min

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March 04, 2022

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,
1Neuroscience Department,Wellesley College

Capitoli

  • 00:04Introduction
  • 00:34Prepare the Micro-Incinerator
  • 01:02Sterilize the Pick, Spatula, or Scalpel
  • 01:37Comparative Method: Sterilization of Instruments Using a Bunsen Burner
  • 02:20Experiment
  • 03:19Results: Bacterial Counts Across Sterilization Methods
  • 04:03Conclusion

Summary

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This article describes a method for sterilizing worm picks, spatulas, and scalpels using a micro-incinerator in place of an open flame.

Tags

Flame-free SterilizationC. ElegansPicksSpatulasScalpelsMicro-incineratorBunsen BurnerAseptic TechniqueOP50 E. ColiBacterial Solution

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