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Thermochemical Studies of Ni(II) and Zn(II) Ternary Complexes Using Ion Mobility-Mass Spectrometry

Article DOI: 10.3791/63722-v • 16:11 min • June 8th, 2022
June 8th, 2022 •
1, 1, 1, 1, 1, 2, 1
1Department of Chemistry, Texas A&M University-Commerce, 2Laboratoire de Chimie Théorique, Sorbonne Université

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This article describes an experimental protocol using electrospray-ion mobility-mass spectrometry, semi-empirical quantum calculations, and energy-resolved threshold collision-induced dissociation to measure the relative thermochemistry of the dissociation of related ternary metal complexes.

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Thermochemical Studies Ni(II) Zn(II) Ternary Complexes Ion Mobility-mass Spectrometry Molecular Modeling Reaction Dynamics Theory Dissociation Reactions Reactants Products Conformational Structures Ligand's Affinity Metal Ion Peptide Tags Recombinant Protein Purification Metal Chelation Nitrilotriacetic Acid Immobilized Metal Affinity Column Blunt Nose Syringe Electrospray Flow Rate Microliters Per Minute Negative Ion Mobility-mass Spectrometry (IM-MS) Mode Mass-to-charge Isotope Pattern Amb-metal-NTA Complex
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