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Immunology and Infection

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Intravital Widefield Fluorescence Microscopy of Pulmonary Microcirculation in Experimental Acute Lung Injury Using a Vacuum-Stabilized Imaging System

Article DOI: 10.3791/63733-v • 09:28 min • April 6th, 2022
April 6th, 2022 •
1, 2, 2,3, 4, 5, 6,7, 2,4, 1,2,4,8
1Department of Physiology and Biophysics, Dalhousie University, 2Department of Microbiology and Immunology, Dalhousie University, 3Department of Neuroscience, Dalhousie University, 4Department of Anesthesia, Pain Management, and Perioperative Medicine, Dalhousie University, 5Live Cell Imaging Center, University of Calgary, 6Department of Physiology and Pharmacology, University of Calgary, 7Department of Biochemistry and Molecular Biology, University of Calgary, 8Department of Pharmacology, Dalhousie University

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Intravital fluorescence microscopy can be utilized to study leukocyte-endothelial interactions and capillary perfusion in real-time. This protocol describes methods to image and quantify these parameters in the pulmonary microcirculation using a vacuum-stabilized lung imaging system.

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Intravital Widefield Fluorescence Microscopy Pulmonary Microcirculation Acute Lung Injury Vacuum-stabilized Imaging System Surgical Preparation Imaging Window Vacuum Grease Glass Cover Slip Vacuum Pump Digital Pressure Gauge Fiber Optic Cable Endotracheal Cannula Lidocaine HCL Anesthesia Modified Otoscope Intubation Mouse Ventilation
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