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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins
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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Article DOI: 10.3791/63889-v • 09:09 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1,7, 1, 1, 2, 2, 3, 4, 5, *6, *1
1Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, 2Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 3Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 4Department of Chemical Engineering, Imperial College London, 5Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), 6Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, 7Institute of Biotechnology, Czech Academy of Sciences, BIOCEV
* These authors contributed equally

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Septins are cytoskeletal proteins. They interact with lipid membranes and can sense but also generate membrane curvature at the micron scale. We describe in this protocol bottom-up in vitro methodologies for analyzing membrane deformations, curvature-sensitive septin binding, and septin filament ultrastructure.

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In Vitro Methods Ultrastructural Organization Membrane Reshaping Curvature Sensitivity Septins Cytoskeletal Filamentous Proteins Scanning Electron Microscope Fixation Methods Nanometer Scale Organization Fundamental Research Norland Optical Adhesive NOA Replica Polydimethylsiloxane Undulated Patterns Photopolymerize Polymer Film Air Plasma Cleaner Hydrophilic Surface Small Unilamellar Vesicles SUVs
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