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Facilitating Cerebral Organoid Culture via Lateral Soft Light Illumination

Article DOI: 10.3791/63989-v • 09:10 min • June 6th, 2022
June 6th, 2022 •
1,2, 1,2, 1,2, 1,2, 3, 1,2
1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences, Wuyi University

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Cerebral organoids provide unprecedented opportunities for studying organ development and human disease pathology. Although great success has been achieved with cerebral organoid culture systems, there are still operational difficulties in applying this technology. The present protocol describes a cerebral organoid procedure that facilitates medium change and organoid transfer.

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Cerebral Organoid Culture Lateral Soft Light Illumination Brain Organoids Medium Change Methods Organoid Transfer Operations Automatic Cultures Machines IPSCs MTeSR1 Medium Matrigel Petri Dish PBS Cell Detachment Solution Single Cells EB Formation Medium Centrifuge Anti Adherence Rinsing Solution Rho Kinase Inhibitor Y27632 Specialized 24 Well Plate
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