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JoVE Journal Biology
Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells
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Isolation, Characterization, and Therapeutic Application of Extracellular Vesicles from Cultured Human Mesenchymal Stem Cells

Article DOI: 10.3791/64135-v • 07:03 min • September 23rd, 2022
September 23rd, 2022 •
1,2, 2,3, 2,4, 2,5, 2,5, 2, 2, 2,3
1College of Life Science, Northwest University, 2State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, Fourth Military Medical University, 3Xi'an Institute of Tissue Engineering and Regenerative Medicine, 4School of Basic Medicine, Fourth Military Medical University, 5Department of Orthodontics, School of Stomatology, Fourth Military Medical University

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The present protocol describes the differential centrifugation for isolating and characterizing representative EVs (exosomes and microvesicles) from cultured human MSCs. Further applications of these EVs are also explained in this article.

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Isolation Characterization Therapeutic Application Extracellular Vesicles Cultured Human Mesenchymal Stem Cells Differential Centrifugation Translation Of Research Modern Disease Treatments Video Presentation Culture Medium Alpha-MEM Centrifuge Tubes Cell Fragments Cellular Debris Ultracentrifuge Tubes Microvesicle Pellet PBS
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