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Journal / Bioengineering / A "Plug-And-Display" Nanoparticle Vaccine…
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Bioengineering

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A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Article DOI: 10.3791/64213-v • 08:07 min • July 25th, 2022
July 25th, 2022 •
1, 1, 1, 1, 1, 1, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University

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The present protocol describes the bioengineering of outer membrane vesicles to be a "Plug-and-Display" vaccine platform, including production, purification, bioconjugation, and characterization.

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Nanoparticle Vaccine Outer Membrane Vesicles SARS-CoV-2 Receptor-binding Domain Antigen-specific Immune Response Vaccine Platform Display Antigens Biological Nanoparticles Nano-sized Carriers Cytolysin A Spy Catheter Transformation Plasma Solution BL 21 Competent Strain Luria Bertani Medium Agar Plate Ampicillin OMV Spycatcher Production
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