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JoVE Journal Behavior
Measuring Skeletal Muscle Thermogenesis in Mice and Rats
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Measuring Skeletal Muscle Thermogenesis in Mice and Rats

Article DOI: 10.3791/64264-v • 07:56 min • July 27th, 2022
July 27th, 2022 •
1, 1, 2, 3, 3, 3, 3, 1, 1, 1,3, 1,3
1School of Biomedical Sciences, Kent State University, 2College of Public Health, Kent State University, 3Department of Biological Sciences, Kent State University

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Summary
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Mice and rats are surgically implanted with remote temperature transponders and then habituated to the testing environment and procedure. Changes in muscle temperature are measured in response to pharmacological or contextual stimuli in the home cage or during prescribed physical activity (i.e., treadmill walking at a constant speed).

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S Visible. Apply Gentle Pressure To Ensure That The Transponder Is Securely Implanted In The Muscle Tissue. Release The Pressure And Remove The Applicator. Repeat This Process For Each Mouse Or Rat Being Tested.Once All Animals Have Been Implanted With Transponders Place Them In A Temperature-controlled Environment And Allow Them To Acclimate For At Least 30 Minutes. Use A Temperature Scanner To Measure The Baseline Muscle Temperature Of Each Animal. Record The Readings In A Spreadsheet Or Data Collection Software.Apply The Desired Pharmacological Stimulation Such As An Injection Of A Drug Or Exposure To A Specific Environmental Condition. Monitor The Muscle Temperature At Regular Intervals Using The Temperature Scanner. Record The Readings And Analyze The Data To Determine Changes In Skeletal Muscle Thermogenesis.Repeat The Experiment With Different Conditions Or Interventions As Needed. This Protocol Provides A Reliable And Efficient Method For Measuring Skeletal Muscle Thermogenes
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