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High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
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High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Article DOI: 10.3791/64364-v • 14:03 min • March 24th, 2023
March 24th, 2023 •
1, 2, 3, 1, 1
1Frankel Cardiovascular Regeneration Core Laboratory, Cardiovascular Medicine-Internal Medicine, University of Michigan, Ann Arbor, 2CAIRN Research, 3StemBioSys, Inc.

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Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) offer an alternative to using animals for preclinical cardiotoxicity screening. A limitation to the widespread adoption of hiPSC-CMs in preclinical toxicity screening is the immature, fetal-like phenotype of the cells. Presented here are protocols for robust and rapid maturation of hiPSC-CMs.

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High-Throughput Cardiotoxicity Screening Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers Protocol Researchers Commercial HiPSC-CMs In-house-made HiPSC-CMs Adult-like Phenotype Predictive Value High Throughput Format Optical Mapping Calcium Change Voltage Change Disease Mechanisms Drug Screening Jeffrey Creech Laboratory Maturation-inducing Extracellular Matrix (MECM) Plates Hank's Balanced Salt Solution (HBSS) Cardiomyocyte Plating Liquid Nitrogen Tank Thawing Cells 70% Ethanol Plating Medium
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