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JoVE Journal Bioengineering
In vitro Saggi di cleavage utilizzando Drosophila caspasi ricombinanti purificate per lo screening del substrato
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In vitro Saggi di cleavage utilizzando Drosophila caspasi ricombinanti purificate per lo screening del substrato

Article DOI: 10.3791/64392-v • 08:16 min • October 6th, 2022
October 6th, 2022 •
1, 1
1Department of Molecular, Cell and Cancer Biology, UMass Chan Medical School

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Qui presentiamo un protocollo per esprimere e purificare la Drosophila caspases ricombinante Dronc e Drice e il loro uso nei saggi di clivaggio in vitro .

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Bioingegneria Numero 188
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