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Developmental Biology

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JoVE Journal Developmental Biology
An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
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An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)

Article DOI: 10.3791/64445-v • 06:26 min • September 7th, 2022
September 7th, 2022 •
1,2, 1,2,3, 1
1Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, 2College of Life Science, University of Chinese Academy of Sciences, 3Innovative Academy of Seed Design, Chinese Academy of Sciences

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The tomato seed is an important model for studying genetics and developmental biology during plant reproduction. This protocol is useful for clearing tomato seeds at different developmental stages to observe the finer embryonic structure.

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Efficient Clearing Protocol Study Seed Development Tomato Solanum Lycopersicum L. Sodium Hypochlorite Treatment Observation Of Tomato Embryos Chemical Preparation Harvesting Fruits Days After Flowering (DAF) Ice Early Fruits Middle Fruits Late Fruits Fresh Seeds Collection Precision Forceps Stereo Microscope FAA Fixative Ethanol Dehydration Clearing Solution Cover Slip Incubation
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