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Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans
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Using a Combination of Indirect Calorimetry, Infrared Thermography, and Blood Glucose Levels to Measure Brown Adipose Tissue Thermogenesis in Humans

Article DOI: 10.3791/64451-v • 04:54 min • June 2nd, 2023
June 2nd, 2023 •
1,3, 1, 1, 1, 1,2
1La Trobe Institute for Molecular Science, Department of Rural Clinical Sciences, La Trobe University, 2School of Biomedical Sciences, Department of Physiology, The University of Melbourne, 3Melbourne Medical School, Department of Rural Health, The University of Melbourne

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Here, we present a protocol to quantify the physiological significance of the impact of brown adipose tissue (BAT) activity on human metabolism. This is achieved by combining carbohydrate loading and indirect calorimetry with measurements of supraclavicular changes in temperature. This novel approach can help develop a pharmacological target for BAT thermogenesis in humans.

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Indirect Calorimetry Infrared Thermography Blood Glucose Levels Brown Adipose Tissue Thermogenesis Carbohydrate Loading Supraclavicular Temperature Ionizing Radiation Core Temperature Reference Region Height And Weight Measurement Baseline Parameters Carbohydrate Drills Caffeine Treatment Capsules Respiratory Gas Analyzer Silicone Mask Non-rebreathing Valve Room Air Delivery Mesh Attachment Leaks Detection Expired Gases Sampling
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