JoVE Journal
Biochemistry
Biochemistry
È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.
Capitoli
Riepilogo
In this work, a high-throughput, intracellular calcium fluorescence assay for 384-well plates to screen small molecule libraries on recombinant G protein-coupled receptors (GPCRs) is described. The target, the kinin receptor from the cattle fever tick, Rhipicephalus microplus, is expressed in CHO-K1 cells. This assay identifies agonists and antagonists using the same cells in one "dual-addition" assay.
