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Detection of Abnormal Prion Protein by Immunohistochemistry
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Detection of Abnormal Prion Protein by Immunohistochemistry
DOI:

06:38 min

May 05, 2023

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Capitoli

  • 00:04Introduction
  • 00:48Deparaffinization and Rehydration
  • 01:39Epitope Retrieval
  • 02:31Inactivation of Endogenous Peroxidase and Immunodetection
  • 04:59Results: Epitope Demasking for Proper PrP Immunolabeling and Minimizing Non-Specific Background Staining
  • 06:00Conclusion

Summary

Traduzione automatica

Immunolabeling abnormal prion protein using immunohistochemistry protocols requires specific sample and anti-PrP antibody preparation methodologies. The present protocol describes the key steps in epitope demasking to ensure proper PrP immunolabeling and to minimize non-specific background staining. Also, this approach considers biosafety measures when conducting immunohistochemistry studies with the prion-infected tissues.

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