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Journal / Cancer Research / Intra-Cardiac Injection of Human Prostate Cancer…
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Cancer Research

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JoVE Journal Cancer Research
Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model
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Intra-Cardiac Injection of Human Prostate Cancer Cells to Create a Bone Metastasis Xenograft Mouse Model

Article DOI: 10.3791/64589-v • 06:32 min • November 4th, 2022
November 4th, 2022 •
*1,2, *1,2, 1,2, 1,2, 1,2, 1,2, 1,3, 1,2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 3Institute of Chinese Traditional Surgery, Longhua Hospital, Shanghai University of Traditional Chinese Medicine
* These authors contributed equally

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Here, we present a protocol for the intra-cardiac injection of human prostate cancer cells to generate a mouse model with bone metastasis lesions.

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Intra-cardiac Injection Human Prostate Cancer Cells Bone Metastasis Xenograft Mouse Model Natural Progress In Vivo Molecular Mechanisms Therapeutic Effects Luciferase-labeled PC3 Cells Cell Culture Dish Sterile PBS Trypsinize F12 Medium Cell Concentration Centrifuge Cell Pellet Surgery Room Cell Injection
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